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. 2013 Oct;87(20):11019–11030. doi: 10.1128/JVI.03031-12

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Fig 6 — Staufen-1 is a binding partner of the HERV-K(HML-2) Gag protein. (A) Coimmunoprecipitation of FLAG–Staufen-1 with codon-optimized HERV-K113 Gag-V5 (28) using anti-V5-Sepharose. (Top) Coprecipitated FLAG–Staufen-1 detected by anti-FLAG. (Middle) Precipitated HERV-K(HML-2) Gag-V5. An anti-HERV-K(HML-2) capsid antiserum from rat was used for detection. The star marks the mouse light chain IgG band. (Bottom) The presence of FLAG–Staufen-1 in the lysates was verified. (B) Colocalization of Rec-GFP, HERV-K(HML-2) Gag-Cherry, and FLAG–Staufen-1 stained with anti-FLAG and anti-rabbit IgG-Alexa 647 in HEK 293T cells expressing oriHERV-K113. Small spots of colocalization are indicated by white arrows (see enlargement). No colocalization of HERV-K(HML-2) Gag with Staufen-1 is visible in the assumed budding structures at the cell membrane.