Fig 2.

Lytic susceptibility of Burkitt lymphoma cells to known lytic cycle-inducing agents increases in the presence of AG490. (A and B) HH514-16 cells were treated for 48 h with NaB (A) or for 72 h with Aza (B) with or without AG490. Lytically infected cells were detected using reference human sera by flow cytometry (dotted line, vehicle-treated cells; solid line, NaB- or Aza-treated cells; bold line, NaB/Aza- and AG490-treated cells). Numbers in solid boxes represent the percentage of lytic cells in NaB- or Aza-treated cells compared to vehicle-treated cells; numbers in bold boxes represent the percentage of lytic cells in NaB/Aza- and AG490-treated cells compared to vehicle-treated cells. (C and D) Flow cytometric enumeration of cells expressing EA-D, an early lytic protein (C), or gp350, a late lytic protein (D), using specific MAbs following treatment of cells with NaB with or without AG490 for 24 or 48 h. (E) Intracellular levels of STAT3 in cells grown in the presence of vehicle (solid line) or AG490 (dashed line) for 48 h. Experiments were performed three times; error bars represent standard errors of the means. ∗, P < 0.05 by one-way analysis of variance with post hoc Bonferroni analysis.