Fig 3.

The rate of fusion with the WT HSV glycoproteins is constant over a wide time frame. Two sets of plasmids (DSP or RLuc8) were used to measure fusion rates over a short and a long time course using the WT versions of each HSV glycoprotein. (A and B) The rates measured over the same period for cells transfected with DSP plasmids (A) and cells transfected with RLuc8 plasmids (B) are similar. The sensitivity is markedly enhanced with RLuc8 plasmids. (B and C and D) Raw data are shown for comparison. RLuc8 plasmids yield robust luminescence readings taken every 5 min for 2 h (C) or every minute for 30 min (D).