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. 2013 Nov;87(21):11659–11669. doi: 10.1128/JVI.01434-13

Fig 6.

Fig 6

PABP1 precipitates with N but not NSs or NSm. (A) Immunofluorescence assay of nuclear NSs and PABP1. (B) Western blot analysis of lysates of NSs-transfected 293T cells. Lysates were precipitated with antibodies specific for V5. Blots were probed for PABP1, V5, and GAPDH. (C) Western blot analysis of lysates of NSm-transfected 293T cells. Lysates were precipitated with antibodies specific for FLAG. Blots were probed for PABP1, FLAG, and GAPDH. (D) Western blot analysis of lysates of N-transfected 293T cells. Lysates were precipitated with antibodies specific for N. Blots were probed for PABP1, N, and GAPDH. (E) Western blot analysis of lysates of RVFV MP12-infected and mock-infected HeLa cells harvested 18 h postinfection. Lysates were precipitated with antibodies specific for RVFV N. Blots were probed for PABP1, RVFV N, and GAPDH as a loading control.