Fig 7.

SiRNA knockdown of PABP1. (A) Western blot analysis of lysates of cells transfected with siRNA specific for PABP1 or a control protein (1, nucleolin; 2, 14-3-3 epsilon; 3, GAPDH), nontargeting siRNA, or transfection reagent alone or of untreated cells. Blots were probed for PABP1 or for calnexin as a loading control. (B) Flow cytometry plots of cells stained for PABP1 and RVFV N. Top panels, cells were uninfected; bottom panels, cells were MP12 infected (24 h infection); left panels, cells were treated with PABP1 siRNA; right panels, cells were treated with nontargeting siRNA.