Fig 6.

(A to H) Immunophenotyping of splenocytes from infected WT and MDA5^−/− mice. Mice were infected with 10² PFU of WNV in the footpad. Splenocytes were harvested and analyzed by flow cytometry at 7 days after infection. Numbers (A to D) and percentages (E to H) of the indicated populations are shown; symbols represent individual mice. CD8⁺, IFN-γ⁺, and TNF-α⁺ populations represent cells that were restimulated with an immunodominant WNV peptide. (I to K) Representative flow cytometry plots of IFN-γ⁺, TNF-α⁺, and granzyme B⁺ cell populations. Numbers indicate the percentage of cells in each quadrant.