Fig 7.

(A to F) Immunophenotyping of brain leukocytes in infected WT and MDA5^−/− mice. Mice were infected with 10² PFU of WNV in the footpad. Brains were harvested and analyzed by flow cytometry at 7 days after infection. Numbers (A to C) and percentages (D to F) of the indicated populations are shown; symbols represent individual mice. CD8⁺, IFN-γ⁺, and TNF-α⁺ populations represent cells that were restimulated with an immunodominant WNV NS4B peptide. (G and H) Representative flow cytometry plots of IFN-γ⁺ and TNF-α⁺ populations. Numbers indicate the percentage of cells in each quadrant.