Table 2.
Phenotypes of splenocytes in WT and MDA5−/− mice after WNV infectiona
| Phenotype | Total no. of cells |
P | % of gated cells |
P | Type of gated cell | ||
|---|---|---|---|---|---|---|---|
| WT | MDA5−/− | WT | MDA5−/− | ||||
| CD4+ | 2.3 × 107 (2.2 × 106) | 2.3 × 107 (2.0 × 106) | 0.86 | 16.8 (0.7) | 16.9 (1.1) | 0.98 | Splenocytes |
| CD4+ CD25+ FoxP3+ | 2.5 × 106 (3.3 × 105) | 2.6 × 106 (2.8 × 105) | 0.78 | 10.2 (0.6) | 11.6 (0.6) | 0.12 | CD4+ T cells |
| CD8+ | 4.1 × 106 (3.4 × 105) | 3.6 × 106 (3.8 × 105) | 0.30 | 9.9 (0.8) | 9.9 (0.9) | 0.99 | Splenocytes |
| IFN-γ+ | 3.2 × 105 (4.8 × 104) | 2.4 × 105 (4.5 × 104) | 0.23 | 7.4 (0.9) | 6.5 (0.9) | 0.47 | CD8+ T cells |
| TNF-α+ | 2.0 × 105 (4.3 × 104) | 1.5 × 105 (2.8 × 104) | 0.15 | 5.8 (0.7) | 4.7 (0.4) | 0.18 | CD8+ T cells |
| Granzyme B+ | 4.0 × 105 (5.8 × 104) | 4.6 × 105 (9.3 × 104) | 0.55 | 10.3 (1.0) | 13.1 (1.6) | 0.14 | CD8+ T cells |
a
Mice were infected with 102 PFU of WNV in the footpad. Splenocytes were harvested and analyzed by flow cytometry at day 7 after infection. Numbers and percentages of the indicated populations are shown as means (SEMs) of approximately 15 mice per group. CD8+, IFN-γ+, and TNF-α+ populations represent cells that were restimulated with an immunodominant WNV peptide.