Table 3.
Phenotypes of antigen-specific CD8+ T cells in the spleens of WT and MDA5−/− mice after WNV infectiona
| Phenotype | Fluorescence intensity within IFN-γ+ cells |
P | Fluorescence intensity within TNF-α+ cells |
P | ||
|---|---|---|---|---|---|---|
| WT | MDA5−/− | WT | MDA5−/− | |||
| KLRG1 | 102.2 (3.5) | 132.0 (5.2) | 0.00005* | 100.3 (3.2) | 117.9 (7.9) | 0.044* |
| PD1 | 102.9 (4.4) | 123.3 (9.1) | 0.05 | 96.3 (3.0) | 108.3 (6.8) | 0.11 |
| CD43 | 104.5 (6.8) | 110.4 (8.7) | 0.59 | 100.4 (5.9) | 92.1 (5.2) | 0.30 |
| CD62L | 103.3 (4.5) | 112.8 (6.7) | 0.24 | 111.0 (9.2) | 138.5 (9.7) | 0.049* |
a
Mice were infected with 102 PFU of WNV in the footpad. Splenocytes were harvested and analyzed by flow cytometry at 7 days after infection. Values indicate the geometric mean fluorescence intensity of the indicated phenotypic marker within IFN-γ+ or TNF-α+ populations, shown as means (SEMs) of approximately 15 mice per group. Asterisk indicates significant differences between WT and MDA5−/− cells.