. 2013 Nov;87(21):11401–11415. doi: 10.1128/JVI.01403-13

Table 5.

Phenotype of antigen-specific CD8⁺ T cells in the brains of WT and MDA5^−/− mice after WNV infection^a

Phenotype	Fluorescence intensity within CD8⁺ cells		P	Fluorescence intensity within IFN-γ⁺ cells		P	Fluorescence intensity within TNF-α⁺ cells		P
Phenotype	WT	MDA5^−/−	P	WT	MDA5^−/−	P	WT	MDA5^−/−	P
IFN-γ⁺	98.4 (4.9)	107.0 (5.1)	0.24
TNF-α⁺	94.2 (6.3)	98.5 (7.4)	0.67
KLRG1				100.2 (5.8)	109.1 (6.2)	0.31	99.4 (6.0)	104.0 (5.3)	0.57
PD1				102.6 (5.2)	140.1 (13.1)	0.02*	101.7 (5.3)	146.4 (15.7)	0.02*
CD43				100.3 (4.9)	112.7 (8.8)	0.24	104.7 (6.1)	116.6 (8.1)	0.25
CD62L				96.0 (8.5)	122.9 (14.8)	0.14	104.1 (14.7)	125.9 (15.5)	0.32

Mice were infected with 10² PFU of WNV in the footpad. Brains were harvested and analyzed by flow cytometry at 7 days after infection. Values indicate the geometric mean fluorescence intensity of the indicated activation or phenotypic marker within CD8⁺, IFN-γ⁺, or TNF-α⁺ populations, shown as means (SEMs) of 8 mice per group. Asterisk indicates significant differences between WT and MDA5^−/− cells.