Fig 1.

Effect of the packaging signal on the incorporation efficiency of recombinant NP vRNA in the presence or absence of the other seven vRNAs. (A) Schematic diagram of the recombinant NP vRNA. The noncoding (NCR) and coding (CR) regions are represented by gray and white boxes, respectively; the dashed lines indicate nucleotides deleted from the NP coding region. The GFP ORF was inserted in frame with the NP ORF, as indicated by the black boxes. (B and C) The incorporation efficiencies of the recombinant NP vRNAs were determined as described in Materials and Methods in the presence (eight segments) (B) or absence (one segment) (C) of the remaining seven vRNAs. Statistical analysis was performed using a paired Student t test.