Fig 3.
The protease activity of FMDV 3Cpro is required for disruption of microtubule organization. (A) Vero cells expressing active FMDV 3Cpro (i to iii) or inactive 3Cpro (iv to vi) fused to mCherry (red) were fixed, permeabilized, and immunostained for α-tubulin (green). (B) Vero cells expressing active FMDV 3Cpro (i to iii) or inactive 3Cpro (iv to vi) fused to mCherry (red) were fixed, permeabilized, and immunostained for γ-tubulin (green). Nuclei were visualized with DAPI (blue). Cells expressing 3Cpro are indicated with dashed boxes and shown at high magnification (iii and vi). The arrow in panel B, vi, indicates MTOC. Nuclei were visualized with DAPI (blue). (C) Control Vero cells (CT) or Vero cells expressing active FMDV 3Cpro or inactive 3Cpro fused to mCherry were lysed. Total cell lysates were analyzed by Western blotting to detect Cherry tag, α-tubulin, γ-tubulin, and β-actin.