Fig 4.
The protease activity of FMDV 3Cpro is required for inhibition of microtubule regrowth. Vero cells expressing inactive FMDV 3Cpro (A) or active 3Cpro (B) fused to mCherry (red) were incubated with nocodazole to depolymerize microtubules. Cells were washed and incubated in the absence of nocodazole to initiate microtubule regrowth. Cells were fixed, permeabilized, and immunostained for α-tubulin (green) at the indicated times. The regions of interest of selected 3C-positive (a) and 3C-negative (b) cells at each time point are shown at high magnification on the right of each panel (gray) to show microtubule asters at MTOC.