Fig 7.

SPR analysis of nondimerized Ad3 fiber knob interactions with DSG2. (A) DSG2 was immobilized on sensorchips, and background was automatically subtracted from the control flow cell. The Ad3 fiber knobs (without a dimerization domain, termed noDD) were injected for 3 min at 2.5 μg/ml, followed by a 2.5-min dissociation period. (B) Summary of SPR data. A concentration range from 2.5 to 10 μg/ml of the knobs was injected, and kinetics and affinity parameters were evaluated using BIAeval software. The extracted data are shown in the table. Wt, Ad3 fiber knob without mutations.