Fig 5.

The UL36USP C40A mutation reduces the replication of recombinant virus in HEK293T cells. (A) Schematic diagram of construction of the C40A HSV-1 BAC. Cys40 of UL36 was substituted for by Ala in the HSV-1 genome. Confluent Vero cells were infected with the indicated viruses at MOI of 0.1 (B) and 1.0 (C). Growth curves were generated by luciferase activity assays (B) and traditional plaque assays (C). (D, E, and F) HEK293T cells were infected with WT HSV-1 or C40A HSV-1 at an MOI of 0.1. Growth curves were generated by luciferase activity assays. (D) The expression of ICP0, UL42, and UL46 was detected by Western blotting. (E) The transcription levels of ISG54 and ISG56 were detected by semiquantitative PCR (F).