Fig 3.
Dengue virus blocking in Wolbachia-infected Drosophila lines deficient for Toll and Imd pathways. (A) DENV-2 was injected into 2-day-old female flies, spz2wMelPop and relE20wMelPop, and their tetracycline-treated uninfected counterparts, spz2tet and relE20tet. (B) DENV-2 was injected into 2-day-old females, spz4wMelPop and w1118wMelPop control flies, and their tetracycline-treated counterparts, spz4tet and w1118tet. Total DENV-2 in whole female Drosophila (n = 13 to 15) was measured 6 days postinjection by qRT-PCR using a TaqMan assay specific to DENV in 1 μg of total RNA. The fraction of flies that had detectable DENV-2 infections is shown above each set of data points. Mann-Whitney U test; ****, P < 0.0001.