Skip to main content
. 2013 Nov;87(21):11751–11761. doi: 10.1128/JVI.01809-13

Fig 3.

Fig 3

T cell responses to late SVV genes in BAL fluid are primarily mediated by CD4 T cells during acute infection. The frequency of IFN-γ-producing CD4 (black) and CD8 (gray) T cells in BAL fluid and PBMC in response to immunogenic SVV ORFs was measured by intracellular cytokine staining (ICS) following stimulation with the indicated overlapping peptide pools. (A) Representative example of the CD8 T cell response directed against ORF4 in BAL fluid. (Left panel) Delineation of CD4 and CD8 T cells. (Middle panel) CD8 T cell response following stimulation with 0.05% DMSO. (Right panel) CD8 T cell response following stimulation with overlapping peptide pool of ORF4. (B and C) Average percentages ± SEM of responding IFN-γ+ T cells against listed ORFs in BAL fluid. (D and E) Average percentages ± SEM of responding IFN-γ+ T cells against listed ORFs in PBMC. (F and G) Average percentages of responding IFN-γ+ T cells against putative immediate early/early (IE/E) or late (L) ORFs in BAL fluid (F) and PBMC (G). *, P < 0.05.