Fig 9.

Uncleaved gp140 trimer from isolate 92UG37 does not exhibit functional gp120-gp41 interactions. (A) Plots comparing the exchange profiles of 92UG37 monomeric gp120 versus foldon-stabilized, trimeric gp140. Individual exchange plots are shown for a fragment from gp120 layer 1 (B) and the large fragment spanning the C-terminal end of gp120 to the end of HR1 (C). Numbering in parentheses refers to HXB2 numbering. Plots comparing the exchange profile with or without sCD4 are shown for monomeric gp120 (D) and trimeric gp140 (E) are also shown. Individual exchange plots for each fragment are shown in Fig. S3 in the supplemental material.