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. 2013 Nov;87(21):11912–11915. doi: 10.1128/JVI.01680-13

Fig 1.

Fig 1

General structures of modified HIV-1 genomes. (A) GagBSL-BCherry is similar to the previously described construct GagCeFP-BglSL (19) but contains a Bgl-mCherry fusion protein in nef; the translation of Bgl-mCherry is directed by an IRES from EMCV. (B) GagNoSL-BCherry is similar to GagCeFP-BCherry but lacks the recognition sites (BSL) of the BglG protein. (C) NoGagBSL-BCherry is similar to GagBSL-BCherry but contains two mutations in gag to prevent Gag translation, an AAG substitution in place of the Gag translational start codon AUG, and a 4-bp insertion in CA that results in a premature stop codon in CA. (D) The NC domain of GagLZBSL-BCherry was replaced with a leucine zipper (LZ) motif. Open boxes, HIV-1 sequences; blue boxes, CeFP coding sequences; red stem-loops, recognition sites of the BglG protein (BSL); red boxes, mCherry coding sequences.