Fig 8.
Growth kinetics of SIVmac239 and various HIV-1mt clones in TRIM5α homozygous RhM PBMCs. PBMCs were prepared from four RhM individuals with the different TRIM5 alleles indicated. Viruses were prepared from 293T cells transfected with the indicated proviral clones, and equal amounts (2.5 × 106 RT units) were used to spin infect PBMCs (2 × 106 cells). As a control experiment, rhesus M1.3S cells (2 × 105) were infected with equal amounts of viruses (5 × 105 RT units). Virus replication was monitored by RT activity released into the culture supernatants. Monkey identification numbers are indicated in each panel.