Fig 1.
Schematic representation of the FMDV genome organization and cDNA expression cassettes for FMDV capsid proteins. Plasmids are based on pGEM-3Z and possess an ampicillin cassette for selection. The P1-2A, P1-2A-3C, and P1-2A-mIRES-3C FMDV cDNA cassettes have been described elsewhere (22, 31). P1 and P1-2A, capsid precursor proteins; 3C, 3Cpro wt or C142S; mIRES, internal ribosome entry site with the GTTA mutant motif; VP1 K210E, VP1/2A cleavage site mutant; ATG, start codon; TGA, stop codon; UTR, untranslated region. The two forms of the L protein, termed Lab and Lb, are made by translation initiation at separate AUG codons 84 nt apart.