Fig 2.
Expression and 3Cpro processing of FMDV capsid protein precursor P1, P1-2A, or P1-2A (VP1 K210E). Plasmids containing the FMDV serotype O (A and B) or serotype A (C and D) cDNA cassettes were transfected into BHK cells infected with recombinant vaccinia virus vTF7-3. Cytoplasmic extracts were prepared 20 h posttransfection and fractionated by SDS-PAGE, followed by transfer to PVDF membrane and probing with antibodies specific for FMDV capsid proteins (A and C), FMDV 2A protein (B and D), and β-actin (bottom panels). Expression of β-actin was used as a control of equal protein loading. The results shown are representative of two independent experiments. Molecular mass markers (kDa) are indicated in the left margin.