Fig 3.
Detection of expressed FMDV capsid proteins using serotype-specific anti-FMDV antibodies and anti-2A antibodies. (A to C) Samples were prepared using serotype O cDNA cassettes as described in the legend to Fig. 2 and analyzed using different anti-FMDV antigen ELISAs as described in Materials and Methods. FMDV proteins captured by rabbit anti-FMDV antibodies were detected directly with guinea pig anti-FMDV antibodies (A), captured with guinea pig anti-FMDV antibodies and detected with anti-2A antibodies (B), or captured by the αvβ6 integrin and detected with guinea pig anti-FMDV (C). (D to F) Similarly, samples were prepared using serotype A cDNA cassettes and analyzed using the different FMDV antigen ELISAs in which antibody-captured FMDV proteins were detected directly with anti-FMDV antibodies (D) or with anti-2A antibodies (E) or following capture by the αvβ6 integrin and detection with guinea pig anti-FMDV antibodies (F). The results shown are representative of two independent experiments. Samples were analyzed in the same order as described in the legend to Fig. 2. Abs, absorbance; AU, absorbance units.