Table 1.
Primers
| Primer namea | Sequenceb (5′–3′) |
|---|---|
| 1-O PN 3 Fw* | AACCACTCAACGGAGAGTGGAA |
| 1-V PN 48 Re* | ACTGTACTGTGYRWAGTACTGGGC |
| 8-A PN 51 Fw* | CCACAGATCAAGGTGTATGC |
| 1-O PN 20 Re* | GACATGTCCTCCTGCATCTG |
| FMDVOP1_AscIXmaI_Re | CTACCCGGGGGCGCGCCTGGGCCCAGGGTTGGACTCCACATCTC |
| FMDVAP1_AscIBamHI_Re | CTAGGATCCGGCGCGCCTGGGCCCGGGGTTGGACTCAACGTCTCCT |
| FMDVA_VP1K210E_Fw | CCTGCAGAACAACTTTTGAACTTCGATTTGCTCAAGTTG |
| FMDVA_VP1K210E_Re | AAGTTGTTCTGCAGGTGCAATGATCTTCTGTTTGTGTCT |
| FMDVO_VP1E83K_Fw | AAGCACAAGGGAAACCTCACCTGGGTCCCGAACGGGGCG |
| FMDVO_VP1E83K_Re | GTTTCCCTTGTGCTTCACTGCCACCTCTAAGTCAGCGAA |
| FMDVO_VP1C134S_Fw | GGGAACAGCAAGTATGGTGACGGCACGGTGGCCAATGTG |
| FMDVO_VP1C134S_Re | ATACTTGCTGTTCCCGTTGTAAACAGTAGCCAACACGCG |
| FMDVO_NheIVP4VP2_Fw | CGCTCTGCTAGCCGACAAGAAAACCGAGGAGACC |
| FMDVO_ApaI2A2B_Re | CTACTAGGGCCCAGGGTTGGACTCCACATCTCCC |
a
Asterisks indicate primers used to amplify parts of the P1-2A coding region of FMDV O1 Manisa (Lindholm).
b
Underlined sequences represent restriction enzyme sites AscI, BamHI, XmaI, NheI, and ApaI. Nucleotide changes producing amino acid substitutions are shown in boldface.