Fig 2.

Type I IFN receptor controls late but not early resistance of FDC to M protein-mediated inhibition of host gene protein synthesis. The rates of host protein synthesis were measured in wt and IFNAR^−/− FDC after infection with wt VSV. Wild-type and IFNAR^−/− FDC were infected with wt VSV (MOI of 10) and pulse labeled with [³⁵S]methionine at the indicated times postinfection, and the rates of host protein synthesis were evaluated by gel electrophoresis, phosphorimaging, and densitometry. (a) Representative gel containing lysates from wt and IFNAR^−/− FDC. Arrow indicates M protein. (b) Cumulative results expressed as percentages of synthesis in the mock-infected control. Data represent means ± SD from at least 3 independent experiments per group. P values for IFNAR^−/− FDC compared to wt FDC are as follows: *, P = 0.467; **, P = 0.006; ***, P = 0.001. (c) Protein synthesis in wt GDC. (d) Cumulative results from multiple experiments. Host protein synthesis is expressed as percentage of the synthesis in the mock-infected control. (e) Protein synthesis in L929 cells; viral proteins are indicated with arrows.