Fig 5.

FDC maturation in response to wt VSV is partially dependent on MyD88. FDC prepared from wt or MyD88^−/− mice were infected with wt or M51R VSV at the indicated MOI. Controls were mock treated or treated with 1 mM loxoribine (lox). Maturation of the total DC population (>90% CD11c⁺) gated on live cells was evaluated by flow cytometry after staining for CD86. (a) Representative histograms from wt-VSV-infected cells. Top, wt FDC; bottom, MyD88^−/− FDC. (b) Representative histograms from M51R VSV-infected or lox-treated cells. Top, wt FDC; bottom, MyD88^−/− DC. (c and d) Cumulative data from multiple experiments showing fold increases in CD86 MFI for wt-VSV-infected cells, M51R VSV-infected cells, or lox-treated cells. Mock-infected FDC did not significantly increase CD86 expression over the course of the 24-h culture period relative to levels expressed at start of culture (data not shown).