Fig 2.
Mutations that disrupt nuclease activity do not affect mitochondrial localization. Transfected HeLa cells were costained with rabbit anti-FLAG (red) and mouse anti-cytochrome c (green) and visualized using fluorescence microscopy. Colocalizations of SPA-tagged proteins (containing three FLAG epitopes) with the mitochondrial protein cytochrome c are indicated in yellow. For clarity, the DAPI channel has been omitted. Scale bars, 10 μm.