Fig 6.

Knockdown of ENDOG and EXOG using siRNA. (A) Endogenous EXOG knockdown was measured in HeLa cells transfected with the indicated siRNAs. (B) Lysates from HeLa cells transfected with the indicated siRNAs were separated into cytoplasmic (C) and mitochondrial (M) fractions to visualize knockdown of EXOG in the mitochondrial fraction. (C and D) Alternatively, HeLa cells were cotransfected with the indicated siRNAs and ENDOG-myc (C) or EXOG-myc (D) for 48 h to verify siRNA function. EXOG, ENDOG-myc, and EXOG-myc levels were quantitated following immunoblotting and infrared detection. Data from three separate experiments were averaged, plotted relative to actin or MnSOD, and normalized to the N.C. siRNA treatment. Standard errors are indicated.