Table 1.

Generic and clade-specific primers

PCR no.	Round no.	Primer sequence		Product size (kb)	Target gene	Targeted herpesvirus(es)	Annealing temp (°C)
		Sense	Antisense
1	I	CGCAAATCGCAGA(N/I)KC(N/I)TGGTG	TGGTTGCCCAACAG(N/I)ATYTCRTT	0.32	gB	Members of the genus Cytomegalovirus	45
	II	TTCAAGGAACTCAGYAARAT(N/I)AAYCC	CGTTGTCCTC(N/I)CC(N/I)ARYTG(N/I)CC	0.25			45
2	I	CCTGTCGCACAATGTGGACATG	CAGCTGTTTTCCGAA(N/I)GTTTCRTTAT	0.25	UL56	Members of the genus Cytomegalovirus	45
	II	TGGCCTACGCYTGYGAYAACG	GCGAACGTGC(N/I)TCCACATCTCC	0.18			45
3	I	CGTGAAATGTGCCGAGGGAACG	TCCGACATAACGGGCCGCGA	2.2	UL56-gB	WRC cytomegalovirusesa	68
	II	GGATGGTCGTCAAGTATCAAGGCTTTT	GGCCGCGATCGGTTTGTCGTA	2.2			68
4	I	GCTCATCCGTTGGTGGCCTTCC	GCAACTGGGCATAAACCACACTTTGA	0.58	gB	WRC cytomegalovirusesa	58
	II	ACTACAGCATGGTGGATTCCTACGG	CATCAGCCAGTTGCTTCATCTCCC	0.39			58
5	I	GAYTTYGC(N/I)AGYYT(N/I)TAYCC	5′-GTCTTGCTCACCAG(N/I)TC(N/I)AC(N/I)CCYTT	0.48–0.71	DPOL	Members of the family Herpesviridae	46
		TCCTGGACAAGCAGCAR(N/I)YSGC(N/I)MT(N/I)AA
	II	TGTAACTCGGTGTAYGG(N/I)TTYAC(N/I)GG(N/I)GT	5′-CACAGAGTCCGTRTC(N/I)CCRTA(N/I)AT	0.21–0.235			46
6	I	ACGGCCTCTTCCCCTGCCTA	GCAGCTGGCCTTCGGGGTTG	0.165	DPOL	WRC lymphocryptovirusesa	62
7	I	CCTCCCAGGTTCARTWYGCMTAYGA	CCGTTGAGGTTCTGAGTGTARTARTTRTAYTC	0.55	gB	Members of the genus Rhadinovirus	45
	II	AAGATCAACCCCAC(N/I)AG(N/I)GT(N/I)ATG	GTGTAGTAGTTGTACTCCCTRAACAT(N/I)GTYTC	0.5			45
8	I	GCCAAGTTCGTGGGAGACGCC	CGCACGTCTCCTTGCACGTCT	0.23	gB	WRC and BWC rhadinovirusesa	62

^aIdentified in this study.