Fig 1.

Identification of the interaction between the Myc-tagged UL105 and FLAG-tagged Snapin by co-IP. Human U251 cells were cotransfected with a combination of two plasmids expressing FLAG- and Myc-tagged proteins. Cellular lysates were prepared at 48 h posttransfection. The input protein samples (70 μg; Input, lanes 3, 6, 9, and 12) and samples (10 μg) that were immunoprecipitated with either anti-Myc [IP(anti-Myc), lanes 2, 5, 8, and 11] or anti-FLAG [IP(anti-FLAG), lanes 1, 4, 7, and 10] antibodies were separated on SDS-containing polyacrylamide gels and analyzed with anti-Myc (anti-Myc) and anti-FLAG (anti-FLAG) antibodies, respectively.