Fig 4.

Identification of the interaction between UL105 and different FLAG-tagged Snapin mutants by co-IP. Human U251 cells were cotransfected with pCMV-UL105 and a plasmid expressing FLAG-tagged Snapin protein with different mutations. Cellular lysates were prepared at 48 h posttransfection. The input protein samples (50 μg) (Input, lanes 1 to 8) and samples (10 μg) that were immunoprecipitated with anti-UL105 [IP(anti-UL105), lanes 9 to 16) were separated on SDS-containing polyacrylamide gels and analyzed with anti-FLAG antibodies (anti-FLAG).