Fig 8.

Effect of altered expression of Snapin and its mutants on the nuclear and cytoplasmic distribution of UL105. Different cells (e.g., parental U251 cells, cells overexpressing Snapin and its mutants [U251-S, U251-ΔH1, and U251-ΔH2]) were either transfected with anti-Snapin H2-siRNA molecules (lanes 1 to 3 and 8 to 10) or not transfected with any siRNAs (lanes 4 to 7 and 11 to 14). Cells were infected with HCMV (MOI = 1) at 48 h transfection, harvested at 48 to 72 h postinfection, and separated into nuclear and cytoplasmic fractions. (Top three panels) Equivalent amounts of each fraction were analyzed by immunoblotting with anti-UL105 and anti-UL70, while the purity of the nuclear and cytoplasmic fractions was assayed by immunoblotting with anti-histone H1 and antiactin, respectively. (Bottom) The analyses were repeated three times, and the standard deviations are indicated by the error bars.