Skip to main content
. 2013 Oct;87(19):10628–10640. doi: 10.1128/JVI.01657-13

Table 1.

Plasmid constructs used in the study

Plasmid Description Source
pGADT7 Cloning vector for protein expression fused with the GAL4 activation domain in yeast Clontech
pGBKT7 Cloning vector for protein expression fused with the GAL4 DNA-binding domain in yeast Clontech
pCMV-Myc Cloning vector for protein expression fused with c-Myc tag in mammalian cell Clontech
pCDNA3.1(+) Cloning vector for protein expression without a tag in mammalian cells Clontech
pCMV6-XL5-Snapin pCMV6-XL5 containing Snapin cDNA full-length sequence OriGene
pGBKT7-UL105 pGBKT7 containing HCMV UL105 full-length sequence This study
pACT2-SNAPIN/UL105 pACT2 containing full-length human Snapin cDNA sequence This study
pCMV-UL105 pCDNA3.1(+) containing full-length HCMV UL105 sequence This study
pCMV-Myc-UL105 pCMV-Myc containing HCMV UL105 full-length sequence This study
pRK11-FLAG-UL83 pRK11-FLAG containing full-length HCMV UL83 sequence This study
pGADT7-Snapin/UL105 pGADT7 containing full-length human Snapin cDNA sequence This study
pGADT7-SΔHD(1-20aa) pGADT7 containing a truncated Snapin cDNA sequence with deletion of the sequence coding for the region from amino acid residues 1 to 20 This study
pGADT7-SΔL1(20-36aa) pGADT7 containing a truncated Snapin cDNA sequence with deletion of the sequence coding for the region from amino acid residues 20 to 36 This study
pGADT7-SΔH1(37-65aa) pGADT7 containing a truncated Snapin cDNA sequence with deletion of the sequence coding for the region from amino acid residues 37 to 65 This study
pGADT7-SΔL2(66-80aa) pGADT7 containing a truncated Snapin cDNA sequence with deletion of the sequence coding for the region from amino acid residues 66 to 80 This study
pGADT7-SΔH2(81-126aa) pGADT7 containing a truncated Snapin cDNA sequence with deletion of the sequence coding for the region from amino acid residues 81 to 126 This study
pGADT7-SΔCD(127-136aa) pGADT7 containing a truncated Snapin cDNA sequence with deletion of the sequence coding for the region from amino acid residues 127 to 136 This study
pRK11-FLAG-Snapin pRK11-FLAG containing full-length human Snapin cDNA sequence This study
pRK11-FLAG-SΔHD(1-20aa) pRK11-FLAG containing a truncated Snapin cDNA sequence with deletion of the sequence coding for the region from amino acid residues 1 to 20 This study
pRK11-FLAG-SΔL1(20-36aa) pRK11-FLAG containing a truncated Snapin cDNA sequence with deletion of the sequence coding for the region from amino acid residues 20 to 36 This study
pRK11-FLAG-SΔH1(37-65aa) pRK11-FLAG containing a truncated Snapin cDNA sequence with deletion of the sequence coding for the region from amino acid residues 37 to 65 This study
pRK11-FLAG-SΔL2(66-80aa) pRK11-FLAG containing a truncated Snapin cDNA sequence with deletion of the sequence coding for the region from amino acid residues 66 to 80 This study
pRK11-FLAG-SΔH2(81-126aa) pRK11-FLAG containing a truncated Snapin cDNA sequence with deletion of the sequence coding for the region from amino acid residues 81 to 126 This study
pRK11-FLAG-SΔCD(127-136aa) pRK11-FLAG containing a truncated Snapin cDNA sequence with deletion of the sequence coding for the region from amino acid residues 127 to 136 This study
pCMV-Snapin/UL105 pCDNA3.1(+) containing Snapin full-length sequence (amino acid residues 1 to 136) This study
pCMV-SΔH1 pCDNA3.1(+) containing a truncated Snapin cDNA sequence with deletion of the sequence coding for the region from amino acid residues 37 to 65 This study
pCMV-SΔH2 pCDNA3.1(+) containing a truncated Snapin cDNA sequence with deletion of the sequence coding for the region from amino acid residues 81 to 126 This study