Fig 1.

Circulating myeloid dendritic cells can be isolated from peripheral blood. (A) Flow cytometric analysis was performed on peripheral blood mononuclear cells stained with FITC-conjugated anti-TCRα/β, -CD14, and -CD19 antibodies (top right) or isotype-matched antibody controls (top left). Following magnetic column depletion with anti-CD3, -CD7, -CD16, -CD56, and -CD123 antibodies, the unbound fraction was stained with FITC-conjugated anti-TCRα/β, -CD14, and -CD19 antibodies (bottom). CD4-positive cells were depleted from the unbound fraction and stained with FITC-conjugated anti-TCRα/β, -CD14, and -CD19 antibodies and a PE-conjugated anti-HLA-DR antibody. (B) Isolated DCs were stained with a panel of cell surface markers (filled histogram) or an isotype-matched control (open histogram) immediately postisolation.