Fig 5.

The MIEP in circulating DCs is predominantly associated with markers of transcriptional activation. (A) Chromatin immunoprecipitation assays were performed on seropositive DCs with rabbit IgG (lane 2), anti-dimethylated histone H3-K4 (lane 3), anti-acetylated H4 (lane 4), anti-rat IgG (lane 5), or anti-HP1β (lane 6) antibodies. DNA amplified in an MIEP-specific PCR was then analyzed by Southern blotting using an MIEP-specific probe. Alternatively, samples were amplified using primers against the HS4 region of the gamma globulin gene. (B) Monocytes isolated from the same donor were either subjected to ChIP (lanes 1 to 3) or differentiated to MoDCs and then subjected to ChIP analysis (lanes 4 to 6). Immunoprecipitation with IgG (lanes 1 and 3), anti-HP-1 (lanes 2 and 5) or anti-acetylated histone H4 (lanes 3 and 6) was performed, and samples were amplified in an MIEP PCR and analyzed by Southern blotting.