Fig 2.

Transcription and expression kinetics of ac83. (A) RT-PCR analysis of ac83 transcription. Total RNA was extracted from AcMNPV-infected cells at the designated time points and was used for RT-PCR analysis. A DNA marker is shown on the left. (B) Time expression profile of Ac83. Cells were either mock infected (mi) or infected with vAc83:HA at an MOI of 5 TCID₅₀s/cell. At different hours p.i., cells were collected and were prepared for Western blotting. Transferred proteins were detected with an anti-HA antibody. A protein marker is shown on the left. (C) Transcriptional mapping of the initiation site of ac83 using 5′ RACE analysis. The AcMNPV genome sequence and 5′ RACE sequencing results are shown. The translation start codon of Ac83 is shown in boldface and is designated +1, and the typical baculovirus late gene promoter consensus motif TAAG is also shown in boldface. The arrow indicates the location of the transcription initiation site of ac83.