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Real-time PCR analysis of viral DNA synthesis. Sf9 cells were transfected in triplicate with vAc83KO or vAc38kKO. At the designated time points p.t., total intracellular DNA was isolated, digested with the restriction enzyme DpnI to eliminate input bacmid DNA, and analyzed by real-time PCR. The results are averages for three independent replication assays. Error bars indicate standard deviations.
