Fig 6.

Expression of Ac83 aa 451 to 600 is sufficient to rescue infectious BV production. (A) Abilities of different Ac83 truncation viruses to rescue infectious BV production. Sf9 cells were transfected with the indicated bacmid, and virus propagation was monitored by fluorescence microscopy at the indicated time points p.t. (B) One-step growth curves of the bAc83KO genome repaired with a virus from which the INM-SM, the CBD, or the PR region of Ac83 was deleted (a) or with a virus in which Ac83 was N-terminally truncated (b), C-terminally truncated (c), or both N- and C-terminally truncated (d). Sf9 cells were infected with the indicated viruses at an MOI of 0.1 TCID₅₀/cell, and at the designated time points p.i., the supernatants were harvested and were analyzed for the release of infectious BVs by a TCID₅₀ endpoint dilution assay. Data points are averages of titers derived from three independent infections. Error bars represent standard deviations.