Fig 8.

Localization of Ac83 in purified and fractionated virions. BVs and ODVs were purified from vAc83:HA-infected Sf9 cells, and the purified ODVs were further fractionated into envelope (E) and nucleocapsid (NC) fractions. Protein samples were subjected to Western blotting in order to determine the localization profile of Ac83 in purified BVs and ODVs (A) or fractionated ODVs (B). Proteins were detected by immunoblotting with an anti-HA antibody to detect HA-tagged Ac83, with anti-ODV-E25 to detect the BV/ODV envelope-associated protein ODV-E25, and with an anti-VP39 antibody to detect the major capsid protein VP39.