Table 2.
Infectivities of vAcWT, vAc83:HA, and vAc83:delCBD in 4th-instar T. ni larvaea and the effect of calcofluor white on the infectivity of vAc83:delCBD
| Inoculation method and virus or control | Dosage | Mortality (%) by 6 days p.i. | LD50b (95% confidence interval) |
|---|---|---|---|
| Injection | |||
| vAcWT | 5 TCID50s | 100 | |
| vAc83:HA | 5 TCID50s | 100 | |
| vAc83:delCBD | 5 TCID50s | 100 | |
| H2O | 4.1c | ||
| Per os | |||
| vAc83:delCBD | 1 × 104 OBs | 8.3 | |
| 5 × 106 OBs | 58.6 | ||
| H2O | 0 | ||
| vAcWT | 123.2 (92.5–164.1) A | ||
| vAc83:HA | 144.9 (108.3–194.1) A | ||
| vAc83:delCBD + calcofluor white | 1.5 × 105 (7.9 × 104-2.7 × 105) B |
a
A total of 24 larvae were used for each treatment.
b
Expressed as OBs/larva. Values followed by different capital letters are significantly different from each other.
c
Larval death not due to virus infection (microscopy and PCR analysis).