Fig 10.
Changes in CD4i antibody binding to gp120 in the presence of sCD4 by SPR. (A) On-rates of N5i5 binding to the four gp120s in the presence and absence of sCD4. (B) On-rates of 17b binding to the four gp120s in the presence and absence of sCD4. (C) Change in rates of association (ka) of 17b and N5i5 with the gp120s as a result of sCD4 binding. Data are presented as the fold increase relative to the rate of association with unliganded gp120. *, the experiment could not be reliably performed. Error bars indicate standard deviations, calculated as described in Materials and Methods.