Fig 8.

Conformation-dependent antibody binding to gp120s as determined by ELISA. Conformation-dependent antibodies specific for different regions of gp120 were tested: CD4 binding site ligands (CD4-IgG2, IgG1-b12, and VRC01), conformational inner domain antibodies (M90, N5i5, and A32), and the coreceptor binding site antibody (17b). Each graph corresponds to a different antibody binding to the four gp120s. The binding curves are color coded based on HIV isolate as shown in the symbol key. Antibody binding levels (measured as the absorbance at 450 nm) versus antibody concentrations are shown. Curves are representative of at least two independent experiments; error bars indicate standard deviations from duplicate measurements.