Table 3.
SPR binding parameters for linear epitope-specific antibodies
| Antibody | Parametera (units) | Affinity of antibody for the gp120 based on SPRb |
|||
|---|---|---|---|---|---|
| 1157ip | 1084i | HXB2 | SF162 | ||
| CA13 | ka (M−1 s−1) | 1.8 (1) × 104 | 1.96 (9) × 104 | 0.9 (1) × 104 | 0.36 (1) × 104 |
| kd (s−1) | 5.31 (4) × 10−4 | 4.3 (2) × 10−4 | 2.03 (1) × 10−4 | 4.32 (9) × 10−4 | |
| KD (nM) | 29 (2) | 22 (2) | 22 (3) | 121 (1) | |
| C4 | ka (M−1 s−1) | 2.6 (2) × 104 | 3.2 (1) × 104 | 1.1 (1) × 104 | 0.42 (5) × 104 |
| kd (s−1) | 3.1 (2) × 10−3 | 1.34 (1) × 10−3 | 1.29 (3) × 10−3 | 2.8 (1) × 10−3 | |
| KD (nM) | 118 (1) | 42 (2) | 110 (10) | 600 (100) | |
a
ka, rate of association; kd, rate of dissociation; KD, equilibrium dissociation constant.
b
Values are means ± standard deviations. Values in parentheses are errors for the last significant figure; 1.9 (1) ×104 is equivalent to 19,000 ± 1,000.