Table 5.
SPR binding parameters for CD4i epitope-specific antibodies
| Antibody | Parametera (units) | SPR-derived binding values for gp120-ligand interactionb |
|||
|---|---|---|---|---|---|
| 1157ip | 1084i | HXB2 | SF162 | ||
| N5i5 | ka (M−1 s−1) | 9.7 (2) × 103 | 31.2 (4) × 103 | 21.2 (6) × 103 | 39.4 (2) × 103 |
| kd (s−1) | —c | — | — | — | |
| KD (nM) | — | — | — | — | |
| N5i5 + sCD4 | ka (M−1 s−1) | 6.1 (2) × 104 | 15.1 (3) × 104 | NDd | 29 (2) × 104 |
| kd (s−1) | — | — | ND | — | |
| KD (nM) | — | — | ND | — | |
| 17b | ka (M−1 s−1) | 4.38 (3) × 103 | 9.5 (1) × 103 | 15.1 (3) × 103 | 50.6 (7) × 103 |
| kd (s−1) | — | 2.72 (1) × 10−5 | — | — | |
| KD (nM) | — | 2.86 (4 | — | — | |
| 17b + sCD4 | ka (M−1 s−1) | 5.5 (1) × 104 | 5.8 (5) × 104 | ND | 41.9 (6) × 104 |
| kd (s−1) | — | 1.3 (1) × 10−4 | ND | — | |
| KD (nM) | — | 2.2 (4) | ND | — | |
a
ka, rate of association; kd, rate of dissociation; KD, equilibrium dissociation constant.
b
Values are means ± standard deviations. Values in parentheses are errors for the last significant figure; see Table 3.
c
—, because of slow dissociation, it was not possible to reliably fit dissociation rates or binding constants for this interaction.
d
ND, could not be reliably performed.