Table 2.
Osmotic-stress-induced changes in transcript levels of genes predicted to encode hydrophilins
| Gene | B728aa |
DC3000a |
||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Locus | AA | Gly | Hyd | Fold changeb | Locus | AA | Gly | Hyd | Fold changeb | |
| hfnA | Psyr_3782 | 63 | 12.7 | 0.89 | 8.83c | PSPTO_1596 | 63 | 12.7 | 0.95 | 2.85d |
| hfnB | Psyr_0768 | 51 | 3.9 | 1.74 | 2.43c | PSPTO_0891 | 76 | 6.6 | 1.14 | 1.76d |
| hfnC | Psyr_0858 | 135 | 10.4 | 0.97 | 2.07c | PSPTO_0993 | 135 | 8.1 | 0.96 | 2.52d |
| hfnD | Psyr_3971 | 156 | 20.5 | 1.22 | Mc | PSPTO_4237 | 152 | 19.7 | 1.16 | 2.50d |
| hfnE | Psyr_0128 | 121 | 8.3 | 0.99 | 1.81c | PSPTO_0281 | 121 | 9.1 | 0.99 | 1.57d |
| hfnF | Psyr_3320 | 49 | 8.2 | 1.97 | Mc | PSPTO_3546 | 47 | 6.4 | 1.93 | (1.17)d |
| hfnG | Psyr_1879 | 51 | 8.9 | 1.83 | 1.70 | PSPTO_2069 | 61 | 8.2 | 1.91 | (1.10) |
| hfnH | Psyr_1972 | 103 | 7.8 | 1.64 | (1.27)c | PSPTO_2162 | 164 | 4.9 | 1.38 | (1.19) |
| hfnI | Psyr_4518 | 183 | 10.4 | 1.14 | (1.13) | PSPTO_0656 | 189 | 10.1 | 1.12 | (−1.24) |
| hfnJ | Psyr_0132 | 82 | 11.0 | 1.29 | M | PSPTO_0274 | 84 | 9.5 | 1.09 | (1.11) |
| hfnK | Psyr_0032 | 176 | 16.5 | 0.98 | (1.09) | PSPTO_0161 | 171 | 17.0 | 1.00 | (−1.03) |
| hfnL | Psyr_4262 | 117 | 9.4 | 0.95 | (−1.15) | PSPTO_4600 | 117 | 9.4 | 0.99 | (1.02) |
Hydrophilins were predicted on the basis of protein size (<200 amino acids), glycine content (≥6% of amino acid content), and estimated hydrophilicity (≥0.95) based on ExPASy's ProtScale (61). Values that did not meet a selection criterion in one of the two strains are in italics. The orthologs of HfnB exhibit distinct sizes because of the absence of an N-terminal domain in Psyr_0768. AA, number of amino acids; Gly, percent glycine content; Hyd, estimated hydrophilicity.
The fold change values in bold had significantly different transcript levels in the presence of NaCl versus in its absence on the basis of an FDR of 1%. For B728a, the fold changes not in bold had significantly different transcript levels on the basis of an FDR of 10%. Values in parentheses did not show significant differences in their transcript levels in response to NaCl and are included for comparison. M, the gene is missing from the B728a microarray.
This gene showed increased transcript abundance in response to osmotic stress in a subsequent transcriptome analysis (19).
This gene showed increased transcript abundance in response to a matric stress (polyethylene glycol [molecular weight, 8,000]), which represents a distinct form of water stress (62).