Table 3.
Comparison of osmoinduced changes in transcript levels based on microarray versus qRT-PCR analyses for selected DC3000 genes
| Locus | Genec | Fold change in transcript levela after: |
||||
|---|---|---|---|---|---|---|
| 15-min osmotic upshock |
60-min osmotic upshock |
|||||
| Microarray | Moderate NaClb | High NaClb | Moderate NaClb | High NaClb | ||
| PSPTO_1633 | ggnA | 5.24 | 3.2 (0.7) | 3.8 (0.4) | 2.3 (0.1) | 4.8 (2.3) |
| PSPTO_4578 | opuCD | 4.68 | 3.1 (0.2) | 1.5 (0.1) | 4.7 (0.7) | 7.0 (2.6) |
| PSPTO_4906 | 4.62 | 5.8 (3.7) | 2.5 (1.7) | 8.5 (0.7) | 4.2 (1.7) | |
| PSPTO_1596 | hfnA | 2.85 | 9.3 (1.1) | 4.2 (0.4) | 5.8 (3.7) | 2.2 (0.3) |
| PSPTO_3125 | glgA | 2.16 | 1.6 (0.0) | 2.9 (1.2) | 2.7 (0.5) | 7.5 (4.6) |
| PSPTO_0359 | glnA1 | −2.94 | −8.3 (1.7) | −2.9 (0.7) | −2.4 (0.9) | −25.3 (2.2) |
| PSPTO_1274 | cspC | −3.29 | −2.5 (1.2) | −1.3 (0.4) | −2.0 (0.4) | −7.6 (0.4) |
a
Fold change in transcript level relative to conditions without hyperosmolarity, as described in Materials and Methods. The values are averages of two replicates (standard deviation).
b
DC3000 cells in ½21CS were subjected to a 15- or 60-min osmotic upshock with a moderate (0.22 M) or high (0.44 M) NaCl concentration.
c
The gene functions are as defined in Table 2 (see also Table S3 in the supplemental material), with the addition of PSPTO_4906, which encodes a putative ferritin/DPS gene, and PSPTO_1274, which encodes a putative cold shock protein.