Fig 1.

Microcolonies of P. aeruginosa PA14 biofilms grown in 24-well cell culture plates demonstrating the native dispersion response. (A) Transmitted-light images showing the presence and absence of interior voids formed within microcolonies of wild-type PA14 and 8 putative enoyl-CoA hydratase mutants. Biofilms of the dspI (PA14_54640) mutant showed no evidence of void formation. All images are shown at the same relative size at ×200 magnification. Scale bars, 50 μm. (B) Quantification of microcolony voids formed as a percentage of the total number of microcolonies observed for biofilms of PA14 and ΔdspI strains.