Fig 2.

dspI is required for native biofilm dispersion. Transmitted-light images (A and B) and confocal laser scanning microscopy images (C) at a magnification of ×500 of P. aeruginosa wild-type and dspI mutant biofilms. The photomicrographs show microcolonies of biofilms grown in modified EPRI medium (A) or 5% LB medium (B and C) for 6 days, with continuous dspI induction in the complemented dspI mutant strain. Microcolonies of the dspI mutant remained solid, whereas wild-type and complemented mutant biofilms showed dispersion. Experiments were completed in triplicate. Scale bars, 50 μm.