Table 1.
Bacterial strains and plasmids
| Strain or plasmid | Description | Reference or source |
|---|---|---|
| Strains | ||
| E. coli | ||
| DH5α | λ− ϕ80dlacZΔM15 Δ(lacZYA-argF)U169 recA1 endA1 hsdR17(rK− mK−) supE44 thi-1 gyrA relA1 | Invitrogen Corp. |
| P. aeruginosa PA14 | ||
| PA14 | Wild type | 26 |
| PA14Δ19740 | PA14 19740::MAR2 × T7; Gmr | 26 |
| PA14Δ26690 | PA14 26690::MAR2 × T7; Gmr | 26 |
| PA14Δ28310 | PA14 28310::MAR2 × T7; Gmr | 26 |
| PA14Δ40640 | PA14 40640::MAR2 × T7; Gmr | 26 |
| PA14Δ40980 | PA14 40980::MAR2 × T7; Gmr | 26 |
| PA14Δ43440 | PA14 43440::MAR2 × T7; Gmr | 26 |
| PA14Δ51110 | PA14 51110::MAR2 × T7; Gmr | 26 |
| PA14ΔdspI | PA14 54640::MAR2 × T7; Gmr | 26 |
| PA14ΔdspI/pMJT | PA14 ΔdspI bearing empty pMJT-1 vector; Gmr Cbr | This study |
| PA14ΔdspI/pMJT-dspI | Complementation of ΔdspI; Gmr Carbr; arabinose inducible | This study |
| PA14/dspI-lacZ | pCTX-dspI-lacZ conjugated into PA14; Tetr | This study |
| PA14/lacZ | pCTX conjugated into PA14; Tetr | This study |
| Plasmids | ||
| pCR2.1-TOPO | TA cloning vector; Kmr Ampr | Invitrogen Corp. |
| pRK2013 | Helper plasmid for triparental mating; mob tra; Kmr | 31 |
| pMJT1 | araC-PBAD casette of pJN105 cloned into pUCP18; Ampr Cbr | 27 |
| pCTX | mini-CTX-lacZ; Tetr | 28 |
| pMJT-dspI | dspI cloned into pMJT1 using primers dspI_NheI_for/dspI_SacI_rev; Cbr | This study |
| pCTX-dspI-lacZ | dspI promoter reporter construct in mini-CTX-lacZ using primers dspI-PROM_XhoI_for/dspI-PROM_EcoRI_rev; Tetr | This study |