Strains |
|
|
E. coli
|
|
|
DH5α |
λ− ϕ80dlacZΔM15 Δ(lacZYA-argF)U169 recA1 endA1 hsdR17(rK− mK−) supE44 thi-1 gyrA relA1
|
Invitrogen Corp. |
P. aeruginosa PA14 |
|
|
PA14 |
Wild type |
26 |
PA14Δ19740 |
PA14 19740::MAR2 × T7; Gmr
|
26 |
PA14Δ26690 |
PA14 26690::MAR2 × T7; Gmr
|
26 |
PA14Δ28310 |
PA14 28310::MAR2 × T7; Gmr
|
26 |
PA14Δ40640 |
PA14 40640::MAR2 × T7; Gmr
|
26 |
PA14Δ40980 |
PA14 40980::MAR2 × T7; Gmr
|
26 |
PA14Δ43440 |
PA14 43440::MAR2 × T7; Gmr
|
26 |
PA14Δ51110 |
PA14 51110::MAR2 × T7; Gmr
|
26 |
PA14ΔdspI
|
PA14 54640::MAR2 × T7; Gmr
|
26 |
PA14ΔdspI/pMJT |
PA14 ΔdspI bearing empty pMJT-1 vector; Gmr Cbr
|
This study |
PA14ΔdspI/pMJT-dspI
|
Complementation of ΔdspI; Gmr Carbr; arabinose inducible |
This study |
PA14/dspI-lacZ
|
pCTX-dspI-lacZ conjugated into PA14; Tetr
|
This study |
PA14/lacZ
|
pCTX conjugated into PA14; Tetr
|
This study |
Plasmids |
|
|
pCR2.1-TOPO |
TA cloning vector; Kmr Ampr
|
Invitrogen Corp. |
pRK2013 |
Helper plasmid for triparental mating; mob tra; Kmr
|
31 |
pMJT1 |
araC-PBAD casette of pJN105 cloned into pUCP18; Ampr Cbr
|
27 |
pCTX |
mini-CTX-lacZ; Tetr
|
28 |
pMJT-dspI
|
dspI cloned into pMJT1 using primers dspI_NheI_for/dspI_SacI_rev; Cbr
|
This study |
pCTX-dspI-lacZ
|
dspI promoter reporter construct in mini-CTX-lacZ using primers dspI-PROM_XhoI_for/dspI-PROM_EcoRI_rev; Tetr
|
This study |