Table 2.
Strain and conditions | Total biomass (μm3/μm2) | Avg biofilm thickness (μm) | Maximum biofilm thickness (μm) |
---|---|---|---|
Continuous dspI induction (5% LB; 6-day-old biofilms) | |||
PA14 | 9.4 ± 3.8 | 13.4 ± 5.0 | 61.9 ± 21.8 |
ΔdspI | 22.0 ± 7.0c | 35.5 ± 11.5c | 127.1 ± 35.6c |
ΔdspI/pMJT-dspI | 6.5 ± 3.9d,e | 10.4 ± 5.1d,e | 64.2 ± 23.0d,e |
ΔdspI/pMJT | 25.4 ± 10.8c | 39.3 ± 16.8c | 97.4 ± 35.7c |
Before dspI induction (EPRI; 5-day-old biofilms) | |||
PA14 | 9.3 ± 2.9 | 9.7 ± 3.4 | 25.7 ± 7.8 |
ΔdspI | 14.8 ± 2.4c | 16.0 ± 2.6c | 43.0 ± 14.0c |
ΔdspI/pMJT-dspI | 14.5 ± 5.6c,f | 16.4 ± 6.0c,f | 38.7 ± 18.0c,f |
ΔdspI/pMJT | 16.7 ± 3.8c | 18.3 ± 4.4c | 38.8 ± 12.1c |
After dspI induction (EPRI; 6-day-old biofilms)b | |||
PA14 | 9.2 ± 4.7 | 10.5 ± 5.4 | 30.23 ± 13.4 |
ΔdspI | 20.2 ± 3.5c | 22.5 ± 3.7c | 53.8 ± 16.3c |
ΔdspI/pMJT-dspI | 6.0 ± 3.8d,e | 7.0 ± 4.3d,e | 20.4 ± 8.3d,e |
ΔdspI/pMJT | 21.8 ± 7.3c | 23.5 ± 8.1c | 40.0 ± 12.4c |
COMSTAT analysis was carried out on biofilms grown in replicate (n = 2) from at least 8 images per replicate.
dspI expression was induced by growth in arabinose-containing culture medium for 24 h.
Significantly different from the wild-type PA14 (P < 0.01), as determined by single-variant ANOVA.
Significantly different from the dspI mutant (P < 0.01), as determined by single-variant ANOVA.
Not significantly different from the wild type (P > 0.01), as determined by single-variant ANOVA.
Not significantly different from the dspI mutant (P > 0.01), as determined by single-variant ANOVA.