Table 2.

Quantitative analysis of biofilm architecture using COMSTAT^a

Strain and conditions	Total biomass (μm3/μm2)	Avg biofilm thickness (μm)	Maximum biofilm thickness (μm)
Continuous dspI induction (5% LB; 6-day-old biofilms)
PA14	9.4 ± 3.8	13.4 ± 5.0	61.9 ± 21.8
ΔdspI	22.0 ± 7.0c	35.5 ± 11.5c	127.1 ± 35.6c
ΔdspI/pMJT-dspI	6.5 ± 3.9d,e	10.4 ± 5.1d,e	64.2 ± 23.0d,e
ΔdspI/pMJT	25.4 ± 10.8c	39.3 ± 16.8c	97.4 ± 35.7c
Before dspI induction (EPRI; 5-day-old biofilms)
PA14	9.3 ± 2.9	9.7 ± 3.4	25.7 ± 7.8
ΔdspI	14.8 ± 2.4c	16.0 ± 2.6c	43.0 ± 14.0c
ΔdspI/pMJT-dspI	14.5 ± 5.6c,f	16.4 ± 6.0c,f	38.7 ± 18.0c,f
ΔdspI/pMJT	16.7 ± 3.8c	18.3 ± 4.4c	38.8 ± 12.1c
After dspI induction (EPRI; 6-day-old biofilms)b
PA14	9.2 ± 4.7	10.5 ± 5.4	30.23 ± 13.4
ΔdspI	20.2 ± 3.5c	22.5 ± 3.7c	53.8 ± 16.3c
ΔdspI/pMJT-dspI	6.0 ± 3.8d,e	7.0 ± 4.3d,e	20.4 ± 8.3d,e
ΔdspI/pMJT	21.8 ± 7.3c	23.5 ± 8.1c	40.0 ± 12.4c

^aCOMSTAT analysis was carried out on biofilms grown in replicate (n = 2) from at least 8 images per replicate.

^bdspI expression was induced by growth in arabinose-containing culture medium for 24 h.

^cSignificantly different from the wild-type PA14 (P < 0.01), as determined by single-variant ANOVA.

^dSignificantly different from the dspI mutant (P < 0.01), as determined by single-variant ANOVA.

^eNot significantly different from the wild type (P > 0.01), as determined by single-variant ANOVA.

^fNot significantly different from the dspI mutant (P > 0.01), as determined by single-variant ANOVA.